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55420

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CLED Agar

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
BROLACIN Agar, Bromothymol-blue Lactose Cystine Agar, Cystine-Lactose-Electrolyte Deficient Agar
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
beef extract, 3 g/L
bromo thymol blue, 0.02 g/L
casein enzymic hydrolysate, 4 g/L
L-cystine, 0.128 g/L
lactose, 10 g/L
peptic digest of animal tissue, 4 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

final pH

7.3±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages

microbiology

suitability

nonselective and differential for Enterococcus spp.
nonselective and differential for Escherichia coli
nonselective and differential for Klebsiella spp.
nonselective and differential for Lactobacillus spp.
nonselective and differential for Proteus spp.
nonselective and differential for Pseudomonas spp.
nonselective and differential for Salmonella spp.
nonselective and differential for Staphylococcus spp.
nonselective and differential for Streptococcus spp.
nonselective and differential for coliforms

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microbe id | utilization test: suitable, microbiological culture: suitable

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microbe id | specific enzyme detection: suitable, microbiological culture: suitable

form

powder

form

powder

form

powder

form

powder

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

composition

agar, 15 g/L , bromo thymol blue, 0.02 g/L , L-cystine, 0.128 g/L , peptic digest of animal tissue, 4 g/L , beef extract, 3 g/L , lactose, 10 g/L , casein enzymic hydrolysate, 4 g/L

composition

agar, 15 g/L , chromogenic mixture, 20.3 g/L , disodium hydrogen phosphate, 3.5 g/L , lactose, 2.5 g/L , monopotassium hydrogen phosphate, 1.5 g/L , neutral red, 0.03 g/L , peptone, special, 5 g/L , sodium chloride, 5 g/L , yeast extract, 3 g/L

composition

agar, 15 g/L , beef extract, 4 g/L , casein enzymic hydrolysate, 4 g/L , chromogenic mixture, 12.44 g/L , peptic digest of animal tissue, 18 g/L

composition

agar, 12 g/L , gelatine peptone, 1 g/L , D(+)-glucose, 1 g/L , phenol red, 0.012 g/L , potassium dihydrogen phosphate, 2 g/L , sodium chloride, 5 g/L

application(s)

clinical testing
environmental
food and beverages, microbiology

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology

application(s)

clinical testing
clinical testing
environmental
food and beverages
veterinary, microbiology

application(s)

clinical testing
environmental
food and beverages, microbiology

General description

CLED (cysteine-, lactose- and electrolyte-deficient) Agar is recommended for isolation, enumeration and identification of urinary pathogens.
CLED Agar is commonly used in bacteriological analysis.

Application

CLED Agar has been used as a non-selective media to perform enantiomeric profiling of quinolones and quinolones resistance gene qnrS in wastewater samples

Preparation Note

Suspend 36.15g in 1000ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)


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Erika Castrignanò et al.
Water research, 175, 115653-115653 (2020-03-26)
Wastewater-based epidemiology (WBE) was applied for the first time in seven cities across Europe with the aim of estimating quinolones consumption via the analysis of human urinary metabolites in wastewater. This report is also the first pan-European study focussed on
B Bogokowsky et al.
The International journal of applied radiation and isotopes, 34(10), 1441-1443 (1983-10-01)
MacConkey agar and Cystine-Lactose-Electrolyte-Deficient (CLED) agar, media widely used in the bacteriological laboratory and recommended for the detection of urinary tract infections, were sterilized by gamma-radiation at a dose of 1.5 Mrad. Both were modified and adapted to radiation sterilization

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