Skip to Content
MilliporeSigma
  • Effects of N source concentration and NH4(+)/NO3(-) ratio on phenylethanoid glycoside pattern in tissue cultures of Plantago lanceolata L.: a metabolomics driven full-factorial experiment with LC-ESI-MS(3.).

Effects of N source concentration and NH4(+)/NO3(-) ratio on phenylethanoid glycoside pattern in tissue cultures of Plantago lanceolata L.: a metabolomics driven full-factorial experiment with LC-ESI-MS(3.).

Phytochemistry (2014-08-02)
Sándor Gonda, Attila Kiss-Szikszai, Zsolt Szűcs, Csaba Máthé, Gábor Vasas
ABSTRACT

Tissue cultures of a medicinal plant, Plantago lanceolata L. were screened for phenylethanoid glycosides (PGs) and other natural products (NPs) with LC-ESI-MS(3). The effects of N source concentration and NH4(+)/NO3(-) ratio were evaluated in a full-factorial (FF) experiment. N concentrations of 10, 20, 40 and 60mM, and NH4(+)/NO3(-) ratios of 0, 0.11, 0.20 and 0.33 (ratio of NH4(+) in total N source) were tested. Several peaks could be identified as PGs, of which, 16 could be putatively identified from the MS/MS/MS spectra. N source concentration and NH4(+)/NO3(-) ratio had significant effects on the metabolome, their effects on individual PGs were different despite these metabolites were of the same biosynthethic class. Chief PGs were plantamajoside and acteoside (verbascoside), their highest concentrations were 3.54±0.83% and 1.30±0.40% of dry weight, on media 10(0.33) and 40(0.33), respectively. NH4(+)/NO3(-) ratio and N source concentration effects were examined on a set of 89 NPs. For most NPs, high increases in abundance were observed compared to Murashige-Skoog medium. Abundances of 42 and 10 NPs were significantly influenced by the N source concentration and the NH4(+)/NO3(-) ratio, respectively. Optimal media for production of different NP clusters were 10(0), 10(0.11) and 40(0.33). Interaction was observed between NH4(+)/NO3(-) ratio and N source concentration for many NPs. It was shown in simulated experiments, that one-factor at a time (OFAT) experimental designs lead to sub-optimal media compositions for production of many NPs, and alternative experimental designs (e.g. FF) should be preferred when optimizing medium N source for optimal yield of NPs. If using OFAT, the N source concentration is to be optimized first, followed by NH4(+)/NO3(-) ratio, as this reduces the likeliness of suboptimal yield results.

MATERIALS
Product Number
Brand
Product Description

Supelco
2,4-D, PESTANAL®, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1000 mg
Estimated to ship on March 24, 2025
$74.50
Sigma-Aldrich
Thiamine hydrochloride, ≥98%, FCC, FG
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
In Stock
Details...
$54.60
1 kg
In Stock
Details...
$169.00
5 kg
In Stock
Details...
$789.00
10 kg
In Stock
Details...
$1,470.00
Supelco
Pyridoxine Hydrochloride (B6), analytical standard
Pricing and availability is not currently available.
Supelco
Thiamine Hydrochloride (B1), analytical standard
Pricing and availability is not currently available.
Supelco
Pyridoxine hydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 mg
In Stock
Details...
$102.00
Supelco
Thiamine hydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$109.00
Sigma-Aldrich
Acetonitrile solution, contains 0.05 % (w/v) ammonium formate, 5 % (v/v) water, 0.1 % (v/v) formic acid, suitable for HPLC
Pricing and availability is not currently available.
Sigma-Aldrich
Acetonitrile solution, contains 10.0% acetone, 0.05% formic acid, 40.0% 2-propanol
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
4 x 4 L
Estimated to ship on March 24, 2025
$1,980.00
Supelco
2,4-D, PESTANAL®, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
In Stock
Details...
$40.50
Sigma-Aldrich
Pyridoxine hydrochloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
10 g
In Stock
Details...
$47.50
25 g
Estimated to ship on March 24, 2025
$71.00
100 g
In Stock
Details...
$242.00
Sigma-Aldrich
Pyridoxine hydrochloride, ≥98% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 g
In Stock
Details...
$59.50
100 g
In Stock
Details...
$193.00
Sigma-Aldrich
Pyridoxine hydrochloride, meets USP testing specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
10 g
In Stock
Details...
$268.00
$235.84
100 g
In Stock
Details...
$1,560.00
$1,372.80
Sigma-Aldrich
Thiamine hydrochloride, meets USP testing specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 g
In Stock
Details...
$77.70
250 g
In Stock
Details...
$551.00
$484.88
Sigma-Aldrich
Thiamine hydrochloride, reagent grade, ≥99% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
In Stock
Details...
$21.00
10 g
In Stock
Details...
$39.80
25 g
In Stock
Details...
$65.30
100 g
In Stock
Details...
$92.60
250 g
In Stock
Details...
$206.00
1 kg
In Stock
Details...
$556.00
Sigma-Aldrich
Thiamine hydrochloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 g
In Stock
Details...
$68.80
100 g
In Stock
Details...
$141.00
Sigma-Aldrich
Thiamine hydrochloride, tested according to Ph. Eur.
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
50 g
In Stock
Details...
$84.30
Supelco
Residual Solvent - Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
3 x 1.2 mL
In Stock
Details...
$98.40
Pyridoxine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
110 mg
In Stock
Details...
$225.00
USP
Pyridoxine hydrochloride, United States Pharmacopeia (USP) Reference Standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
200 mg
In Stock
Details...
$401.00
Thiamine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
10 mg
In Stock
Details...
$225.00
USP
Thiamine hydrochloride, United States Pharmacopeia (USP) Reference Standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 mg
In Stock
Details...
$415.00
Supelco
2,4-D, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mg
Estimated to ship on March 24, 2025
$130.00
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
4 L
In Stock
Details...
$219.00
18 L
In Stock
Details...
$669.00
4 x 4 L
In Stock
Details...
$731.00
$621.35
20 L
In Stock
Details...
$891.00
200 L
Estimated to ship on March 24, 2025
$3,500.00
Sigma-Aldrich
Methanol, BioReagent, ≥99.93%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 L
In Stock
Details...
$103.00
2 L
In Stock
Details...
$161.00
Sigma-Aldrich
Methanol, Laboratory Reagent, ≥99.6%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 L
In Stock
Details...
$94.00
4 L
In Stock
Details...
$215.00
4 x 2 L
In Stock
Details...
$433.00
6 x 1 L
In Stock
Details...
$467.00
$396.95
4 x 4 L
In Stock
Details...
$717.00
Sigma-Aldrich
Methanol, Absolute - Acetone free
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 gal
In Stock
Details...
$163.00
Sigma-Aldrich
Methanol, NMR reference standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 pkg
Estimated to ship on March 24, 2025
$188.00
1 pkg
Estimated to ship on March 24, 2025
$193.00
$38.60