HPLC Analysis of Polyphenols in Nero d′Avola Red Wine on Discovery^® HS C18 (UV 354 nm)

sample/matrix

Nero d’Avola wine sample filtered prior injection through a 0.45 μm nylon membrane

column

Discovery HS C18, 15 cm x 2.1 mm I.D., 3 μm particles (569255-U)

mobile phase

[A] water, pH 3 with fomic acid; [B] Acetonitrile, pH 3 with fomic acid

flow rate

0.2 mL/min

column temp.

30 °C

detector

UV, 354 nm

injection

5 μL