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  • Determination of d-serine and related neuroactive amino acids in human plasma by high-performance liquid chromatography with fluorimetric detection.

Determination of d-serine and related neuroactive amino acids in human plasma by high-performance liquid chromatography with fluorimetric detection.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2006-08-08)
Suzanne L Grant, Yanina Shulman, Philip Tibbo, David R Hampson, Glen B Baker
ABSTRACT

A simple and versatile methodology using high-performance liquid chromatography (HPLC) with fluorimetric detection was developed to simultaneously determine d-serine along with other metabolically related neuroactive amino acids in the glutamatergic system: L-serine, L-glutamate, L-glutamine, and glycine. On-column sensitivity was in the lower picomole range. Of two chiral thiol reagents investigated, amino acid derivatives of o-phthaldialdehyde (OPA) in combination with N-isobutyryl-L-cysteine were found to have consistently higher responses than their corresponding N-tert-butyloxycarbonyl-L-cysteine derivatives. This methodology was applied to the quantitative detection of amino acids in human plasma and lays the foundation for further investigations of the role of neuroactive amino acids in the pathophysiology and treatment of neurological and psychiatric disorders.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-Glutamine, ReagentPlus®, ≥99% (HPLC)
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L-Glutamine, BioUltra, ≥99.5% (NT)
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L-Serine, BioUltra, ≥99.5% (NT)
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Supelco
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L-Serine, Pharmaceutical Secondary Standard; Certified Reference Material
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L-Glutamine, Pharmaceutical Secondary Standard; Certified Reference Material
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Supelco
L-Glutamine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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L-Glutamine
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L-Glutamine, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source
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D-Serine, ≥98% (TLC)
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Supelco
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Glycine, BioXtra, ≥99% (titration)
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Glycine, tested according to Ph. Eur.
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Glycine, meets analytical specification of Ph. Eur., BP, USP, 99-101% (based on anhydrous substance)
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L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture
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SAFC
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Glycine, suitable for electrophoresis, ≥99%
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