D9905

デンハルト溶液, 凍結乾燥粉末

for Northern and Southern blotting, lyophilized powder

• 別名: Denhardt′s Solution For Blotting Technique
• UNSPSCコード: 12161700
• NACRES: NA.25

特性

• グレード: for molecular biology
• 品質水準: 100
• フォーム: lyophilized powder
• テクニック: Southern blotting: suitable
• その他の活性: DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
• 保管温度: 2-8°C

詳細

詳細

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

アプリケーション

Denhardt′s Solution has been used:

• as a component in hybridization buffer for the hybridization of brain sections
• in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISH_PAP-WM) process
• in the preparation of hybridization mixture

Suitable as a blocking Buffer for Southern and Northern blot membranes.

生物化学的／生理学的作用

Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.

構成

Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).

調製ノート

水5mLで復元すると50倍濃縮液となります。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)