A1054

モノクローナル抗ジゴキシン抗体－アルカリホスファターゼ標識 マウス宿主抗体

clone DI-22, purified from hybridoma cell culture

• 別名: モノクロナール抗ジゴキシン
• UNSPSCコード: 12352203
• NACRES: NA.46
• 結合体: alkaline phosphatase conjugate
• application: DB; ELISA (d)
• クローン: DI-22, monoclonal
• テクニック: direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate; direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate; dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)
• citations: 9

特性

• 由来生物: mouse
• 品質水準: 200
• リコンビナント: expressed in mouse cell line
• 結合体: alkaline phosphatase conjugate
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: DI-22, monoclonal
• フォーム: buffered aqueous solution
• テクニック: direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate; direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate; dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)
• アイソタイプ: IgG1
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Digoxin is a cardiac glycoside that is used to treat chronic heart failure.Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody can be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies and peptides. The antibody is specific for digoxin and digoxin-labeled compounds. The product shows strong cross-reactivity with digoxigenin.

特異性

抗体はジゴキシンに特異的であり、ジゴキシゲニンに高親和性を示します。

免疫原

ジゴキシン-KLH。

アプリケーション

Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:10000-1:6000) and dot blot (at 1:20,000 dilutions using digoxigenin-labeled biomolecules). It has also been used for enzyme immunoassay (EIA).

Nested PCR products were hybridized with 5′-end digoxigenin-labeled oligonucleotide probe. ELISA assays were performed to detect the probe by adding monoclonal anti-digoxin antibody alkaline phosphatase (DI-22) diluted 1:1000 in 1 M phosphate-buffered saline, 1% bovine serum albumin, and 0.1% Tween 20 (Ph 7.4). Detection was performed using Sigma Fass-Pnpp substrate tablets.

物理的形状

Solution in 0.05 M Tris buffer, pH 8.0 containing 1% BSA, 50% glycerol, 1 mM MgCl₂ and 15 mM sodium azide

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)